TABLE 1.

Bactericidal activity of hBD-3 against nosocomial strains with defined resistance phenotypes

OrganismStrainResistance phenotypeahBD-3 concnb (μg/ml)
S. aureus 1AMC AMP CIP ERY GEN OXA RIF4
S. aureus 2AMC AMP CIP ERY GEN LVX OXA RIF TET4
S. aureus 3AMC AMP CIP ERY GEN LVX OXA8
S. aureus 4AMC AMP AZM CIP CTX ERY IPM LVX OXA8
S. aureus 5AMC AMP CIP GEN IPM TOB SXT OXA AZM ERY CTX4
S. aureus 6AMP AMC AZM CIP CTX ERY GEN IPM LVX OXA RIF SXT4
E. faecium 1AMC CIP CTX ERY GEN SXT TEC VAN8
E. faecium 2AMP CIP CTX ERY GEN LVX TEC VAN8
E. faecium 3AMP CIP CTX ERY GEN LVX SXT TEC VAN8
E. faecium 4AMP CIP ERY GEN SXT TEC VAN8
E. faecium 5AMP AMC CIP CLI ERY GEN KAN LVX SXT TEC VAN8
E. faecium 6AMP AMC CLI CRO CTX CIP ERY GEN KAN LVX SXT VAN8
P. aeruginosa 1AMP CFZ CIP CTX CXM GEN LVX SXT TET8
P. aeruginosa 2AMP CFZ CIP CTX CXM GEN LVX IPM MEM SXT TET8
P. aeruginosa 3AMP CFZ CTX CIP GEN IPM LVX SXT TET TOB8
P. aeruginosa 4AMP AMC CFZ CRO GEN NIT SXT8
P. aeruginosa 5AMP AMC CTX LVX TET TOB SXT4
P. aeruginosa 6AMP AMC CFZ CIP GEN IPM TOB SXT8
S. maltophilia 1AMK CIP CTX IPM MEM SAM TZP4
S. maltophilia 2AMK CIP CTX IPM MEM SAM TZP4
S. maltophilia 3AMK CIP CTX IPM MEM SAM TZP8
S. maltophilia 4AMK CAZ CIP FEP IPM SAM TZP8
S. maltophilia 5AMK CIP CTX IPM MEM SAM TZP4
S. maltophilia 6AMK CIP CTX IPM MEM SAM TZP4
A. baumannii 1AMP AMC CFZ CIP IPM LVX PIP SXT TET4
A. baumannii 2AMP CFZ CIP LVX PIP SXT4
A. baumannii 3AMP AMC CFZ CIP IPM SXT TET TOB4
A. baumannii c 4AMP CFZ CTX TET4
A. baumannii c 5CFZ CFM CXM MEZ4
A. baumannii c 6AMP CFZ CFM4
  • a AMC, amoxycillin; AMP, ampicillin; CIP, ciprofloxacin; ERY, erythromycin; GEN, gentamicin; OXA, oxacillin; RIF, rifampin; LVX, levofloxacin; TET, tetracycline; AZM, azithromycin; CTX, cefotaxime; IPM, imipenem; TOB, tobramycin; SXT, trimethoprim-sulfamethoxazole; TEC, teicoplanin; VAN, vancomycin; CLI, clindamycin; CRO, ceftriaxone; NIT, nitrofurantoin; CFZ, cefazolin; MEM, meropenem; SAM, ampicillin-sulbactam; TZP, piperacillin-tazobactam; AMK, amikacin; CAZ, ceftazidime; FEP, cefepime; PIP, piperacillin; TOB, tobramycin; MEZ, mezlocillin.

  • b Concentration of hBD-3 sufficient to obtain a bactericidal effect after 1.5 h of incubation in SPB.

  • c Bacterial strains resistant to one or two classes of antibiotics.