TABLE 1.

Cell wall thickness and TRG in the presence of vancomycin

MediumaMu50MI
Cell wall thickness (nm)bTRG (h)cCell wall thickness (nm)TRG (h)
RMg-31.03 ± 3.2716.031.86 ± 2.1315.0
RM (RMg- plus 30 mM d-glucose)53.29 ± 3.013.561.47 ± 7.332.0
RMg- plus 30 mM l-glutamine31.07 ± 1.7517.031.86 ± 3.1712.0
RMg- plus 30 mM N-acetylglucosamine38.13 ± 3.4911.050.80 ± 9.648.0
RM plus 30 mM l-glutamine57.18 ± 3.184.565.36 ± 7.861.5
  • a See Materials and methods.

  • b Thirty cells of each cell culture with nearly equatorial cut surfaces (photo taken by transmission electron microscopy) were measured for the evaluation of cell wall thickness. Results are expressed as means ± standard deviations.

  • c In the presence of 30 mg/liter vancomycin in BHI medium.