TABLE 2.

Catalytic efficiency of ATP-dependent removal

NRTIKcat/Km (M−1 s−1, 104)c for:
Wild-type RTaMutant RTbMutant RT + next dNTP
Tenofovir0.94 ± 0.171.7 ± 0.31.9 ± 0.1
AZT13.5 ± 0.760 ± 1149 ± 4
d4T13.5 ± 2.138 ± 7.114 ± 2
  • a Wild-type RT average Vmax values for removal of tenofovir, AZT, and d4T: 0.037 ± 0.02, 0.45 ± 0.17, and 0.12 ± 0.00 s−1, respectively; average Km values: 3.9 ± 1.9, 3.3 ± 1.5, and 0.9 ± 0.1 μM, respectively.

  • b Mutant RT average Vmax values for removal of tenofovir, AZT, and d4T: 0.020 ± 0.006, 1.7 ± 0.8, and 0.6 ± 0.3 s−1, respectively; average Km values: 2.1 ± 0.4, 4.1 ± 0.9, and 2.2 ± 0.5 μM, respectively.

  • c Values are averages ± standard errors.