TABLE 1.

MRSA strains used for the validation of the multiplex PCR

StrainIsolationClonal typebMLST profileSCCmec type by:
OriginaYrFull characterization (26)Multiplex PCR
UK13136UK1960Archaic3-3-1-1-4-4-16II
BK793Egypt1961Archaic3-3-1-1-4-4-16II
COLUK1965Archaic3-3-1-1-4-4-16II
E2125Denmark1964Archaic3-3-1-12-4-4-16II
PER34Spain1989Iberian3-3-1-1-4-4-16IAIA
HPV107Portugal1992Iberian3-3-1-12-4-4-16IAIA
BK1953USA1995Iberian3-3-1-12-4-4-16IAIA
PER184Spain1991Iberian3-3-1-12-4-4-16IAI
PER88Spain1992Iberian3-3-1-12-4-4-16IAIA
N315Japan1982NY/Japan1-4-1-4-12-1-10IIII
BK2464USA1996NY/Japan1-4-1-4-12-1-10IIII
JP1Japan1997NY/Japan1-4-1-4-12-1-10IIII
ANS46cAustralia19822-3-1-1-4-4-3IIIIII
R35cUSA19872-3-1-1-4-4-3IIIIII
HU25Brazil1993Brazilian2-3-1-1-4-4-3IIIAIIIA
HSJ216Portugal1997Brazilian2-3-1-1-4-4-3IIIAIIIA
HDG2Portugal1993Brazilian2-3-1-1-4-4-3IIIBIIIB
HUSA304Hungary1993Hungarian2-3-1-1-4-4-3IIIIII
HU106Hungary1996Hungarian2-3-1-1-4-4-3IIIIII
BM18USA1989Pediatric1-4-1-4-12-1-10IVIV
PL72Poland1991Pediatric1-4-1-4-12-1-10IVIV
POL3Poland1992Pediatric1-4-1-4-12-1-10IVIV
HDE288Portugal1996Pediatric1-4-1-4-12-1-10IVIV
COB3Colombia1996Pediatric1-4-1-4-12-1-10IVIV
BK2529USA1996Clone Vd3-3-1-1-4-4-3IVIV
BARGII17USA1996Clone Vd3-3-1-1-4-4-3IVIV
  • a UK, United Kingdom; USA, United States.

  • b Clonal types were defined by a combination of three molecular typing techniques: polymorphisms in the vicinity of ClaI::mecA; ClaI::Tn554 insertion patterns, and SmaI PFGE macrorestriction profiles (see Materials and Methods).

  • c Strains ANS46 and R35 were included as controls for SCCmec type III, and they were representatives of an MRSA clone found in Australia and in the United States (5, 6).

  • d Strains BK2529 and BARGII17 are representatives of an MRSA clone detected in hospitals located in the New York metropolitan area characterized by unique ClaI::mecA pattern V (17, 26, 29).