TABLE 1.

Primer sequences used for PCR analysis of In60

PrimerNucleotide sequence (5′ to 3′)Position in published sequence (bp)Related featureAccession numbera
5′-CSGGC ATC CAA GCA GCA AG1190-1206Conserved sequence of 5′-CS integrons M73819
341IRGTA ACC GTT TGT TTG AGT GGG3627-3607Internal sequence of orf513 L06418
5′-CSRCTT GCT GCT TGG ATG CC1206-1190Reverse and complementary sequence of 5′-CS integrons M73819
IRTGT CAT TTT CAG AAG ACG ACT GCA C214-238Belonging to the IR sequence present in class 1 and 2 integrons X72585
785upGGC TGT TTT CAC TCT CG8962-8978Internal sequence of In60 AF174129
sul1 TGA AGG TTC GAC AGC AC1463-1447Internal sequence of sul1 X12869
341ACGC CCA CTC AAA CAA ACG3605-3622Internal sequence of orf513 L06418
IATGGTG ACA AAG AGA GTG CAA CGG6339-6359Reverse and complementary sequence corresponding to blaCTX-M-9 translational starting codon region AF174129
ISTOPATG ATT CTC GCC GCT GAA GCC7195-7175Sequence corresponding to blaCTX-M-9 translational stop codon region AF174129
IATGRCCG TTG CAC TCT CTT TGT CAC6359-6339Reverse and complementary sequence of blaCTX-M-9 translational starting codon region AF174129
341STOPACA TTA GTC GGC CAG CGG5453-5470Internal sequence of orf513 AF174129
O3000SRGGA GCT TAT GCG CTC AAT CG9323-9304Reverse complementary sequence of orf1005 translational stop codon region AF174129
ISTOPRGGC TTC AGC GGC GAG AAT CAT7175-7195Sequence of blaCTX-M-9 translational stop codon region AF174129
341BGAG GCT TTG GTG TAA CCG4073-4056Internal sequence of orf513 L06418
  • a Accession numbers are for the EMBL/GenBank database.