TABLE 4.

Specificity of sensitivity to caspofungin in the SBE deletion mutants

Inhibitor (μg/disk)Mean zone of inhibitiona (mm) ± SD seen on strain
Isogenic control (A)Δ SBE2 (B)Δ SBE22 (C)Δ SBE2 Δ SBE22 (D)
CAS (2.5)4.4 ± 0.45.7 ± 0.0 +6.0 ± 0.1 ++8.7 ± 0.5 +++
Itraconazole (10)3.3 ± 0.03.0 ± 0.0 +2.3 ± 0.0 ++2.4 ± 0.1 +++
Fluconazole (20)6.0 ± 0.03.4 ± 0.1 +9.1 ± 0.4 ++6.3 ± 0.2
Amphotericin B (20)1.3 ± 0.11.1 ± 0.01.0 ± 0.01.7 ± 0.1
Cycloheximide (0.5)11.2 ± 0.26.2 ± 0.211.1 ± 0.210.9 ± 0.5
Trifluoroperazine (10)2.0 ± 0.02.7 ± 0.12.0 ± 0.02.2 ± 0.1
Tunicamycin (20)2.7 ± 0.23.3 ± 0.02.6 ± 0.12.0 ± 0.0
  • a The measurements are based on triplicate samples from three independent experiments, each performed at a different time point. The means and standard deviations of the three experiments, after first averaging the replicates in each experiment, are indicated. Statistical comparisons for each column (as lettered parenthetically) were performed using one-way analysis of variance followed by pairwise comparisons between groups of interest. Symbols: +, P < 0.05 (comparison of A to B); ++, P < 0.05 (comparison of A to C); +++, P < 0.05 (comparison of A to D).