TABLE 3.

Sensitivity of drug-resistant strains of HCMV to benzimidazole ribosides and GCV, as determined by a DNA hybridization assay

Virus strainMean IC50 ± SEM (μM)aAltered geneAmino acid change (s)
1263W94GCVBDCRB
AD1690.08 ± 0.020.31 ± 0.10.7 ± 0.3NAbNA
8805rec10.03 ± 0.011.7 ± 0.12 ± 1UL97Met460Val
9330rec50.13 ± 0.061.6 ± 0.40.7 ± 0.3UL97His520Glu
8702rec2NDd1.8 ± 0.30.75 ± 0.3UL97Ala594Val
9219rec70.2 ± 0.11.8 ± 0.31 ± 0.8UL97Leu595del
XbaF.40.03 ± 0.014.1 ± 0.90.2 ± 0.2UL97AlaAlaCysArg593del
4955rec0.06 ± 0.030.7 ± 0.07NDpolcThr700Ala
1038rB0.04 ± 0.020.24 ± 0.06>20UL89Asp344Glu and Ala355Val
2916rAe2.7 ± 0.60.31 ± 0.040.48 ± 0.12UL97Leu397Arg
  • a That is, the mean values based on a minimum of three independent determinations.

  • b NA, not applicable.

  • c pol, polymerase.

  • d ND, not determined.

  • e DNA sequencing of the entire virus was not performed. The sequence change in the UL97 ORF conferred resistance to 1263W94 when transferred into wild-type strain AD169.