Summary of chemical footprinting data with pleuromutilin derivativesa

23S rRNA positionNo drugTiamulinValnemulinSB-264128Pleuromutilin
A2058++++++ E++++ E+++ E+++ E
A2059++++ E+++ E++ E+
G2505+− P− P− P− P
U2506++− P− P+ P(+) P
U2584+++ P+ P+++ E++
U2585++++ P(+) P++++ E++ P
  • a E, enhancement; P, protection. The extent of modification with DMS, CMCT, or kethoxal is indicated with + symbols, with (+) and − indicating very weak and no modification, respectively.