TABLE 1.

Primers used in this study

PrimerDNA sequenceAmplicon in Fig. 1EMBL accession no., nucleotide positions
DR-FW5′-GGGCAAAGCGCAGCTATTAG-3′AF261825, 13-32
S004-RV5′-CCCGCAGGGTAAGTAATG-3′PCR1AF261825, 3275-3258
S011-FW5′-CGCCGGCTCCAAAGGAAATGG-3′AF261825, 11764-11784
S014-RV5′-AATTTCCTCATCGTCTAGC-3′PCR2AF261825, 14859-14842
S014-FW5′-AGTCTGTGGCATGAAGAA C-3′AF261825, 14521-14539
S022-FW5′-CGCTGCAAGCACAATGATGA-3′AF261825, 18301-18320
S024-RV5′-GGTACGGTATCGCCTAAGTG-3′PCR3AF261825, 21930-21911
S026-FW5′-TCGGGTAATCTCAGCAGAGC-3′AF261825, 25021-25040
int-RV5′-GGGCATGGTGGCTGAAGGACC-3′PCR4AF261825, 27266-27246
5′-CS5′-GGCATCCAAGCAGCAAG-3′AF261825, 27892-27908
3′-CS5′-AAGCAGACTTGACCTGA-3′AF261825, 28900-28884
flo-FW5′-ATGACCACCACACGCCCCG-3′AF261825, 30482-30500
flo-RV5′-CTAGACGACTGGCGACTTC-3′PCR5AF261825, 31696-31678
tetR-FW5′-CTGCTGATCGTGGGTCT-3′AF261825, 32657-32673
tetA-RV5′-TTGCGAATGGTCTGCGT-3′PCR6AF261825, 333797-33781
aadA2-FW5′-GAGCGCCATCTGGAATCAACG-3′AF261825, 28058-28078
ORF5-RV5′-CCGAACGTTCGGAGGCTCCT-3′PCR7AF261825, 39750-39731
S044-FW5′-ACCAGAGAGAGTTATCGAGC-3′AF261825, 41911-41930
DR-RV5′-CACGAAAAGGAGACGATGAGA-3′PCR8AF261825, 44483-44463
strAF5′-AGCAGAGCGCGCCTTCGCTG-3′NC_001740.1, 761-780
strAR5′-CCAAAGCCCACTTCACCGAC-3′NC_001740.1, 1464-1445