TABLE 1.

Bacterial strains and plasmids

Strain or plasmidGenotype or relevant phenotypeaSource or reference
Strains
    P. aeruginosa
        PAO1Wild type5
        MPAO1Wild typeThe University of Washington Genome Center (UWGC)
        PAO5011spuF::Tc13
        PAO5013spuH::Tc13
        MPAO1oprH::Tn-Tc (strain ID 32552)UWGCb
        MPAO1phoP::Tn-Tc (strain ID 7863)UWGC
        MPAO1phoQ::Tn-Tc (strain ID 37038)UWGC
        MPAO1pmrB::Tn-Tc (strain ID 46521)UWGC
        MPAO1PA4774::Tn-Tc (strain ID 50643)UWGC
    E. coli DH5αrecA1 endA1 gyrA96 thi-1 hsdR supE44 Δ(lac)U169 (φ80Δlac)Bethesda Research Laboratories
Plasmids
    pBluescriptSK+Apr, cloning vectorStratagene
    pQF50Apr, lacZ transcriptional vector13
    pAU2pQF50 derivative carrying the PPA4773-lacZ fusionThis study
    pAU6RpQF50 derivative carrying the PoprH-lacZ fusionThis study
    pAU7pQF50 derivative carrying the PPA3552-lacZ fusionThis study