TABLE 2.

Primers used in this studya

PrimersSequence
yne forward5′-CCCCGGTCTCCCATGATTAAAATCTACC-3′ (BsaI site is underlined.)
yne reverse5′-CTAGTCTAGATTACTGACAAGAACAGTCC-3′ (XbaI site is underlined.)
ysa forward5′-CCCCGGTCTCCCATGAATTTATCAAGAAAACATAAGG-3′ (BsaI site is underlined.)
ysaB forward5′-CCCCGGTCTCCCATGCTTAGTTTAAAACTTGCGGC-3′ (BsaI site is underlined.)
ysaBC forward5′-CATGCCATGGGCGCTTGCCTCCTTTATTCC-3′ (NcoI site is underlined.)
ysaC reverse5′-GCTCTAGATTTAAACTAAGCATTTAAGG-3′ (XbaI site is underlined.)
ysa reverse5′-CATGTCTAGACTTTATCTTTCAACAATTTTGTAGTAAACA-3′ (XbaI site is underlined.)
  • a The source or reference for each primer is this work.