TABLE 2.

Ribotyping, susceptibility, SDS-PAGE of outer membrane proteins, and mRNA expression studies for the clinical isolates of P. aeruginosa

Group and isolateRibotypeSusceptibility valuea (μg/ml) for:46-kDa protein presentbmRNA expressionc for:
IPMMEMErtapenemCAZFEPATMoprDampCmexAmexCmexEmexX
Group 1
    AM602J48>32>32>256>256No0.71293.51.80.190.06
FI506H8216412No0.16660.7200.010.95
BK546A>3216>32>32328NDd0.044800.520.070.206.7
HW311A48>32>32>256>256No0.709702.80.170.0119
DB322A3216>32>32>25664No0.172,4003.50.460.2220
AM339E3232>32>32>256>256No0.282905.02.50.6623
BK553A324>3216>2562No0.096100.410.08024
BK501A3216>32>326432No0.099301.44.00.7629
AM609I80.5>322168NoNAe2,6001.90.170.0529
BK545A>3216>32>32324No0.132701.10.870.2432
DB513N22>3286432Yes0.461,5003.12.00.6043
CK575F3216>32>32164No0.561700.882.90.4153
DB537A>3216>32>32162No0.143800.870.20088
Group 2
CK505C20.58424No0.190.701.59.01.113
UL118C816>32284No0.021.30.960.6709.6
CK558D162>324164No0.382.01.00.841.418
UL517E>3232>32166416No0.352.40.900.951.83.8
BK555G324>32284NoNA2.41.60.15031
CK550D82>324416Yes0.276.21.402.873
Group 3a
MD519O2<0.121282YesNA2.11.01.00.2335
MD504P20.252224YesNA1.81.81.80.655.7
CK516R2<0.122212YesNA3.52.61.40.661.1
MD521Q20.252224YesNA3.811.60.671.1
CK503S1<0.122224Yes1.024.01.89.41.74.0
DB504A20.252422Yes0.9437.51.44.40.714.4
Group 3b
MD119M24>32488Yes1.22.72.42.205.7
CK188B24>3281616Yes4.10.313.93.00.435.8
CK140B24>3281632Yes115.52.22.10.216.9
MD104B22>3281616Yes100.412.2160.5516
UL140L24>324168Yes2.45.1139.31.6330
AM128K2416248YesNA3.60.710.290.412.8
MD510C2116122ND0.812.60.972.70.405.0
DB311D22>32244Yes1.81.41.70.771.26.6
  • a MEM, meropenem; CAZ, ceftazidime; FEP, cefepime; ATM, aztreonam; IPM, imipenem.

  • b Determined by SDS-PAGE of outer membrane proteins.

  • c Relative to the expression of P. aeruginosa ATCC 27853, which is assigned a value of 1.

  • d ND, not determined.

  • e NA, not amplifiable.