TABLE 1.

Primer and probe sequences used in this work

OligonucleotideSequencea,b5′ Modification3′ ModificationPurpose
FKS1-F1719CATTGCTGTGGCCACTTTAGNoneNoneSequencing primer
FKS1-R2212GATTTCCATTTCCGTGGTAGCNoneNoneSequencing primer
HS1SN2GCCAAATTGGTTGAATCTTANoneNoneReal-time PCR primer
HS1AN2GTCATGGTCGACAAGTTTCTNoneNoneReal-time PCR primer
T-WTAAAAATCTCTTAAAGACAAAGTCAAGAAAAAANoneNoneWild-type target
T-T1933CAAAAATCTCTTAAAGGCAAAGTCAAGAAAAANoneNoneT1933C mutation target
T-C1934AAAAAATCTCTTAAATACAAAGTCAAGAAGAAAANoneNoneC1934A mutation target
T-C1934TAAAAATCTCTTAAAAACAAAGTCAAGAAGAAAANoneNoneC1934T mutation target
T-T1929AAAAAATCTCTTAAAGACAATGTCAAGAAAAAANoneNoneT1929A SNP target
T-T1929A-T1933CAAAAATCTCTTAAAGGCAATGTCAAGAAAAANoneNoneT1929A and T1933C mutation target
T-T1929A-C1934AAAAAATCTCTTAAATACAATGTCAAGAAGAAAANoneNoneT1929A and C1934A mutation target
T-T1929A-C1934TAAAAATCTCTTAAAAACAATGTCAAGAAGAAAANoneNoneT1929A and C1934T mutation target
FKS1(WT)CGCGAGTTCTTGACWTTGTCTTTAAGAGATCTCGCGFAMDabcylWild-type probe
FKS1(T1933C)CGCGAGTCTTGACWTTGCCTTTAAGAGATCTCGCGHEXDabcylT1933C mutation probe
FKS1(C1934A)CGCGAGCTTCTTGACWTTGTATTTAAGAGATCTCGCGHEXDabcylC1934A mutation probe
FKS1(C1934T)CGCGAGCTTCTTGACWTTGTTTTTAAGAGATCTCGCGHEXDabcylC1934T mutation probe
  • a Probe domains of molecular beacons and target domains of targets are underlined. Bases in sequences of molecular beacons and targets corresponding to mutations in the C .albicans FKS1gene are boldfaced. All sequences are listed in the 5′ direction.

  • b Each of the degenerate probes represents an equimolar mixture of two identical molecular beacons with either A or T at position W.