TABLE 4.

Single nucleotide changes located in intergenic regions

Strain and siteaBase changeLocation
CB1616-d20
    1512174C→G22 nt upstream of MW1361 (hypothetical protein)
    2694054G→A77 nt upstream of MW2528 (acetate-CoA ligase [EC 6.2.1.1]/acetyl-CoA synthetase)
CB1617-d20
    1654504C→T14 nt upstream of rpsU, 30S ribosomal protein S21
    2041052C→G41 nt upstream of MW1875, hypothetical protein, putative transcription regulator, GntR family
    2694054G→A77 nt upstream of MW2528 (acetate-CoA ligase [EC 6.2.1.1]/acetyl-CoA synthetase)
CB1618-d20
    2694054G→A77 nt upstream of MW2528 (acetate-CoA ligase [EC 6.2.1.1]/acetyl-CoA synthetase)
    2705330T→C13 nt upstream of nrdD, anaerobic ribonucleoside triphosphate reductase
  • a Location of mutations in the S. aureus strain MW2 sequence (GenBank accession no. BA000033).