TABLE 2.

Melting temperatures of FKS1 molecular beaconsa

Molecular beaconTm with the following oligonucleotide:
T-WTT-T1933CT-C1934AT-C1934TT-T1929AT-T1929A-T1933CT-T1929A-C1934AT-T1929A-C1934T
FKS1(WT) 63.2 ± 0.5 60.4 ± 0.350.7 ± 0.551.4 ± 0.3 63.0 ± 0.3 60.4 ± 0.351.7 ± 0.551.9 ± 0.3
FKS1(T1933C)52.4 ± 0.3 64.0 ± 0.3 40.5 ± 0.640.2 ± 0.051.5 ± 0.6 63.4 ± 0.3 39.0 ± 0.339.5 ± 0.6
FKS1(C1934A)60.0 ± 0.357.2 ± 0.0 63.0 ± 0.3 57.2 ± 0.058.9 ± 0.356.2 ± 0.0 62.7 ± 0.5 56.0 ± 0.8
FKS1(C1934T)59.4 ± 0.357.4 ± 0.357.5 ± 0.3 64.0 ± 0.3 58.9 ± 0.356.0 ± 0.357.2 ± 0.0 63.7 ± 0.0
  • a Melting temperatures of complementary molecular-beacon-target hybrids are boldfaced.