TABLE 3.

Ki and Ki/Km of wild-type and mutant HIV-1 RT enzymes

HIV-1 RTTenofovir-PPddATPCarbovir-TPDXG-TP
Ki (μM)a (fold)bKi/Km (fold)cKi (fold)Ki/Km (fold)Ki (μM) (fold)Ki/Km (fold)Ki (μM) (fold)Ki/Km (fold)
Wild type0.155 (1.0)0.46 (1.0)0.024 (1.0)0.072 (1.0)0.181 (1.0)1.05 (1.0)0.008 (1.0)0.05 (1.0)
K65R1.040d (6.7)2.67 (5.8)0.173e (7.2)0.441 (6.1)0.357 (2.0)1.42 (1.3)0.033e (4.1)0.13 (2.8)
K65R+M184V0.824d (5.3)1.62 (3.5)0.383d (16)0.690 (9.6)0.807d (4.5)2.45 (2.3)0.033e (4.1)0.10 (2.2)
  • a Ki values are averages for three to six experiments, with average standard errors of 0.067 for tenofovir-PP, 0.013 for ddATP, 0.059 for carbovir-TP, and 0.003 for DXG-TP.

  • b Fold change in Ki value from that for the wild type.

  • c Fold change in Ki/Km value from that for the wild type.

  • d P < 0.001 compared to the Ki for the wild type by two-tailed Student's t test.

  • e P < 0.05 compared to the Ki for the wild type by two-tailed Student's t test.