TABLE 1.

Initial identification and characteristics of mutants

M. smegmatis strainGrowth on isoniazid at 50 μg/mlA405aA600Signal/ growthbGrowth on isoniazid at 42°C
mc21551.3940.2084.629NDc
I64+0.3960.4050.978ND
Tn1+0.2560.2790.918+
Tn2+0.2780.3200.869+
Tn3+0.2860.2880.993
  • a Absorbance of the alkaline phosphatase product in the MSH-capture ELISA (23).

  • b Proportional to the MSH contents of the cells.

  • c ND, not determined.