TABLE 2.

Levels of thiol and amine intermediates and ligase activity in MSH-deficient mutants

M. smegmatis strainGlcN-Ins concn (μmol/g [dry wt])Ligase sp act (nmol/min/mg of protein)MSH concn (μmol/g [dry wt])
mc21550.10.320 ± 0.01010.0
I642.50.004 ± 0.0010.1
Tn12.60.004 ± 0.002<0.004
Tn21.90.004 ± 0.002<0.003