TABLE 1

Validation data for penicillin G in plasma and dried blood spots

ParameterValue(s)
    Penicillin G concn (mg/liter)0.1110100
Plasma
    % matrix effect (± SD); n = 5)85.5 (8.4)106.24 (5.2)110.84 (8.9)94.5 (3.5)
    % process efficiency (± SD); n = 586.4 (5.2)116.2 (8.3)107.8 (3.7)89.8 (6.4)
    % absolute recovery (± SD); n = 5101.5 (6.8)109.6 (9.5)97.7 (8.1)95.8 (5.3)
    Interday variation (RSD%)a; n = 56.335.343.964.79
    Intraday variation (RSD%); n = 156.645.973.745.63
    Accuracy (%); n = 1593.5107.25102.4111.54
    Red cell partition ratio (± SD)b0.98 (±0.02)0.95 (±0.08)1.04 (±0.11)0.96 (±0.11)
    Dried blood spots
    % matrix effect (± SD); n = 5)95.5 (4.3)84.8 (2.9)88.2 (11.1)82.1 (3.3)
    % process efficiency (± SD); n = 5101.5 (5.5)92.5 (4.5)82.6 (2.4)87.1 (5.4)
    % absolute recovery (± SD); n = 5107.1 (8.8)109.1 (4.9)94.5 (10.4)106.3 (7.2)
    Interday variation (RSD%); n = 59.738.378.635.33
    Intraday variation (RSD%); n = 157.337.209.054.13
    Accuracy (%); n = 1511687.592.34101.8
  • a RSD%, percent relative standard deviation.

  • b Calculated using the following formula:

  • partitionratio=(blood[drug]plasma[drug](1hematocrit))hematocrit