TABLE 2.

Km values of wild-type and mutant HIV-1 RT enzymes

HIV-1 RTKm (μM)a
dATPdGTP
Wild type0.3330.172
K65R0.3920.254b
K65R+M184V0.554c0.314c
  • a Km values are averages for multiple experiments, with an average standard error of 0.063 for dATP and 0.03 for dGTP.

  • b P ≤ 0.05 compared to the Km for the wild type by two-tailed Student's t test.

  • c P ≤ 0.01 a compared to the Km for the wild type by two-tailed Student's t test.