TABLE 1

Primers for bisulfite sequencing PCR

Target and primer direction and sequencing roundPrimer sequence, 5′→3′
HBV CpG island II
    Forward, firstTTAGGGTGTGTTGTTAATTGGA
    Reverse, firstTAAAAACCCAAACRACCC
    Forward, secondTGGTTGTTAGGGTGTGTTG
    Reverse, secondACCRACAAATAAAAAAACACAA
HBV CpG island III
    Forward, firstGGAGTGTGGATTYGTATTT
    Reverse, firstTAAAATTTCCCACCTTATAAATC
    Forward, secondAGGTAGGTTTTTTAGAAGAAGAAT
    Reverse, secondAAAAAATACTAACATTAAAATTCCC