TABLE 1.

Comparative efficacies of antimicrobial peptides RP-1 and RP-11 and gentamicin in fluid biomatrices and artificial media under specified assay conditionsa

Biomatrix or medium and peptide or drugIC or MBC (μg/ml)
Coincubation2-h preincubation
IC50IC90MBCIC50IC90MBC
Biomatrices
    Whole blood
        RP-1≤1≤1≤1≤1≤150
        RP-11≤1≤15≤150>50
        Gentamicin≤1≤1≤1≤1≤1≤1
    Plasma
        RP-1≤1≤1≤1≤1≤5>50
        RP-11≤1≤1>50≤1>25>50
        Gentamicin101025101025
    Homologous serum
        RP-1≤1≤1≤1≤1>50>50
        RP-11≤5≤550>50>50>50
        Gentamicin≤1≤1≤1≤155
    PNHS
        RP-1≤1≤5>50≤1≤5>50
        RP-1125>50>50>50>50>50
        Gentamicin≤1≤1≤1≤1≤1≤1
    ΔPNHS
        RP-150>50>50>50>50>50
        RP-1150>50>50>50>50>50
        Gentamicin≤1≤1≤1≤1≤1≤1
Conventional media
    MHB at pH 5.5
        RP-15≤25>5010≤25>50
        RP-11>50>50>50>50>50>50
        Gentamicin55>5055>50
    MHB at pH 7.2
        RP-1≤5≤1050≤5≤1050
        RP-11>50>50>50>50>50>50
        Gentamicin≤1≤1≤1≤1≤1≤1
  • a See Materials and Methods for specific conditions of assay.