TABLE 2.

IC50 and IC90 of RP-1 and RP-11 versus E. coli ML-35 in test biomatrices and MHB estimated from co- and preincubation assay resultsa

Biomatrix or mediumConditionbIC50 (μg/ml)IC90 (μg/ml)
RP-1c
    Whole bloodCo≤1≤1
    Pre≤1≤1
    Homologous plasmaCo≤1≤1
    Pre≤13
    Homologous serumCo≤1≤1
    Pre≤1>50
    MHB at pH 5.5Co615
    Pre1018
    MHB at pH 7.2Co26
    Pre37
RP-11d
    Whole bloodCo≤1≤1
    Pre≤150
    Homologous plasmaCo≤1≤1
    Pre≤130
    Homologous serumCo≤1≤1
    Pre≥50≥50
    MHB at 5.5Co≥50≥50
    Pre≥50≥50
    MHB at 7.2Co≥50≥50
    Pre≥50≥50
  • a See text for detailed assay protocols.

  • b Co, coincubation; Pre, preincubation.

  • c Values for RP-1 were approximated by interpolation of data from Fig. 2A and B.

  • d Values for RP-11 were approximated by interpolation of data from Fig. 2C and D.