TABLE 2.

Plasmids used in this study

PlasmidRelevant characteristicsaSource or reference
pResEmBBNEmr; P15A replicon23
pBluescriptII SK(+)Apr; phagemid cloning vectorStratagene
pResYT10Emr; P15A replicon with the unique PvuII site immediately downstream of erythromycin resistance gene23
pHT1Emr; marker rescue plasmid of 7.0-kb PstI fragment of XcB1 chromosomal DNA which includes downstream region of rmlD gene and four intact rgp genesThis study
pHT2Emr; marker rescue plasmid of 9.8-kb HindIII fragment of XcB2 chromosomal DNA which includes downstream region of gtfC gene and four intact mbr genesThis study
pBSHT1pBluescriptII SK+ containing 4.3-kb PstI fragment which carries of Xc rgp genesThis study
pBSHT2pBluescriptII SK+ containing 7.7-kb HindIII fragment which carries of Xc mbr genesThis study