TABLE 1.

Oligonucleotides used for mutagenesis

OligonucleotideaSequence
G238NNS-15′-CGCCGATAAGACCGGAGCT(AGCT)(AGCT)(GC)GAGCGGGGTGCGCGCG-3′
G238NNS-25′-CGCGCGCACCCCGCTC(GC)(AGCT)(AGCT)AGCTCCGGTCTTATCGGCG-3′
G238Asp-15′-CGCCGATAAGACCGGAGCTGACGAGCGGGGTGCGCGCG-3′
G238Asp-25′-CGCGCGCACCCCGCTCGTCAGCTCCGGTCTTATCGGCG-3′
G238Cys-15′-CGCCGATAAGACCGGAGCTTGCGAGCGGGGTGCGCGCG-3′
G238Cys-25′-CGCGCGCACCCCGCTCGCAAGCTCCGGTCTTATCGGCG-3′
G238Gln-15′-CGCCGATAAGACCGGAGCTCAGGAGCGGGGTGCGCGCG-3′
G238Gln-25′-CGCGCGCACCCCGCTCCTGAGCTCCGGTCTTATCGGCG-3′
G238Pro-15′-CGCCGATAAGACCGGAGCTCCGGAGCGGGGTGCGCGCG-3′
G238Pro-25′-CGCGCGCACCCCGCTCCGGAGCTCCGGTCTTATCGGCG-3′
  • a N represents equal amounts of A, C, G, and T nucleotides. S represents equal amounts of G and C nucleotides.