TABLE 1.

Bacterial strains used in this study

P. aeruginosa strainRelevant genotype or phenotypeSource or reference
K767Prototroph22
K1523K767 mexB; unmarked in-frame deletion10
K1525K767 mexXY; unmarked in-frame deletion10
XY1Spontaneous tigecycline-resistant derivative of K1525This study
XY3Spontaneous tigecycline-resistant derivative of K1525This study
K1542K767 mexB/mexXY; unmarked in-frame deletionsK. Poolea
BXY1Spontaneous tigecycline-resistant derivative of K1542This study
BXY4Spontaneous tigecycline-resistant derivative of K1542This study
K1119K797 mexAB-oprM; unmarked in-frame deletion18
M1Spontaneous tigecycline-resistant derivative of K1119This study
M9Spontaneous tigecycline-resistant derivative of K1119This study
K1455K767 nalB; MexAB-OprM-overproducing strain38
  • a Deletions constructed as for strains K1523 and K1525 (K. Poole, personal communication).