TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionReference or source
E. coli DH5α supE44 hsdR17 recA1 endA1 gyrA96 thi-I relA1 Δ(lacZYA-argF)U169deoR (φ80ΔlacZdM15)Hanahan, 1983
P. aeruginosa strains
    H103Wild-type PAO1Nicas and Hancock, 1980
    H911Wild-type PAKChiron
    H956H911 opmK::miniTn5-TcrChiron
    H957H911 opmA::miniTn5-TcrChiron
    H958H911 opmG::miniTn5-TcrChiron
    H959H911 aprF::miniTn5-TcrChiron
    H960H911 opmI::miniTn5-TcrChiron
    H961H911 opmE::miniTn5-TcrChiron
    H962H911 opmL::miniTn5-TcrChiron
    H963H911 opmD::miniTn5-TcrChiron
    H964H911 opmJ::miniTn5-TcrChiron
    H965H911 opmN::miniTn5-TcrChiron
    H966H911 opmH::miniTn5-TcrChiron
    H967H103 opmF::miniTn5-TcrChiron
    H968H911 opmB::miniTn5-TcrChiron
    H969H911 opmM::miniTn5-TcrChiron
    H730 (K372)PAO6609 (met9011 amiE200 rpsL pvd9) PchPoole et al., 1991
    H743 (K613)K372 oprM::ΩHg; OprM-deficientPoole et al., 1993
Plasmids
    pCR2.1TA cloning vectorInvitrogen
    pJJ104pCR2.1 opmGThis study
    pJJ101pCR2.1 opmHThis study
    pUCP21 Escherichia-Pseudomonas shuttle vectorSchweizer, 1991
    pUCP27 Escherichia-Pseudomonas shuttle vectorSchweizer, 1991
    pJJ106pUCP27 opmGThis study
    pJJ105pUCP27 opmHThis study
    pJJ107pUCP21 opmGThis study
    pJJ109pUCP21 opmHThis study