TABLE 1.

Bacterial strains used in this study

Strain(s)SourceDrug resistanceReference and/or provider
Bifidobacterium longum F8aHuman feces; RRIbTcr 33
Butyrivibrio fibrisolvens JK51aSheep rumen; AustraliaTcrKeith Gregg and Jan Kopečný
Butyrivibrio fibrisolvens 1.230aBovine rumen 1; RRITcr 32
Clostridium sp. strain K10aHuman feces 1; RRITcr 33
Mitsuokella multiacida P208-58aPig feces; JapanTcr 23
Roseburia sp. strain A2-183aHuman feces 2; RRITcr 4
Selenomonas ruminantium FB32, FB34, and FB322aBovine rumen 2; RRITcr 12
Bifidobacterium adolescentis L2-32Human feces 3; RRITcs 4
Bifidobacterium adolescentis L2-32RcSpontaneous Rifr mutant of strain L2-32TcsThis work
Butyrivibrio fibrisolvens 2221RcSpontaneous Rifr mutant of type strain 2221Tcs 21
Mitsuokella multiacida F120RcSpontaneous Rifr mutant of strain A405-1TcsJennifer Martin
Roseburia inulinivorans A2-194RcHuman feces 2; RRI; spontaneous Rifr mutantTcs 4; Jennifer Martin
Selenomonas ruminantium HD4RcSheep rumen; United States; spontaneous Rifr mutantTcs 19
Butyrivibrio fibrisolvens Tc8 to Tc12, Tc21Transconjugants from matings between 1.230 and 2221RTcr 32
Bifidobacterium adolescentis TcKK1 to TcKK5Transconjugants from matings between F8 and L2-32RTcrThis work
Bifidobacterium adolescentis LMG11579dBovine rumen; Gent, BelgiumTcrLiesbeth Masco
Bifidobacterium animalis subsp. lactis LMG11580dChicken feces; Gent, BelgiumTcrLiesbeth Masco
Bifidobacterium bifidum LM588dProbiotic product; Gent, BelgiumTcrLiesbeth Masco
Bifidobacterium pseudocatenulatum LMG11593dSewage; Gent, BelgiumTcrLiesbeth Masco
  • a Isolate for which flanking sequences were studied in detail in this work.

  • b RRI, Rowett Research Institute.

  • c Strain which was used as a recipient in mating experiments.

  • d Only DNA isolated from the strain was used in this study.