TABLE 4.

Differences between caCYP51 and ckCYP51 within 12-Å radius from active sitea

caCYP51ckCYP51Difference (caCYP51ckCYP51)
Phe105Leu113Smaller, remains hydrophobic
Lys119Thr127Loss of positive charge
Ser137Trp145Polar residue changed to bulky hydrophobic
Arg138Lys146Conservative change
Ile304Val306Conservative change
Ala313Ser315Becomes more polar
Val404Ile404Conservative change
Val510Thr512Becomes more polar
  • a None of these changed residues are part of the active site (Fig. 1A).