TABLE 1.

Conjugative transfer of pRAS1 measured by transfer frequencies (proportion of E. coli recipient cells receiving the plasmid) after mating at 22°C for various periods either on a solid surface or in liquid medium

StrainTransfer frequency on the following type of medium after the indicated mating time (h):Broth, 24Transfer frequency ratio for plate mating/ broth mating
Agar
12468142451
24021.2 × 10−34.0 × 10−33.5 × 10−30.130.180.250.480.591.1 × 10−3436
19951.6 × 10−62.5 × 10−63.3 × 10−51.1 × 10−44.1 × 10−46.7 × 10−46.5 × 10−36.8 × 10−28.7 × 10−575