TABLE 2.

Expected fragments in the PCR-RFLP screening for the intI1-blaIMP-4 and 5′ CS-arr-2-aacA4 sequences

Restriction enzymeExpected fragments (bp)
For detection of the intI1-blaIMP-4 (1,008-bp) sequence
    ApaLI96, 912
    BsmAI157, 851
    HinCII57, 268, 288, 395
    HindIII292, 716
For detection of the 5′CS-arr-2-aacA4 (1,182-bp) sequence
    BsoBI73, 350, 759
    BspHI160, 599, 423