TABLE 1.

PCR primers used in this work

Forward and reverse primersSequence 5′-3′Amplicon (size in bp)PositionRemarkReference
int1f imp12CAG AAG ACG GCT GCA CTG AA AGT GTG TCC TGG GCC TGG bla IMP-4 upstream (1,833)−1359 to +474 (blaIMP-4 ATG)Characterization of the upstream region of blaIMP-4 in strain 74510This work
imp11 int3cATG AGC AAA GTT ATC TGT ATT CT AAA GCA GTC TTG ACC TGA bla IMP-4 downstream (2,812)+741 (blaIMP-4 ATG) to 2071 downstream of blaIMP-4Characterization of the downstream region of blaIMP-4 in strain 74510This work
int2a int6bAAC CGA GGA TGC GAA CCA CT CCG AGC CGC TCG TAT AG intI1-arr-2-aacA4-3′CS (3,602)+382 (intI1 ATG) to +482 (orf5 ATG)Characterization of the regions flanking arr-2 in strain 74510This work
int2a imp12AAC CGA GGA TGC GAA CCA CT AGT GTG TCC TGG GCC TGG intI1-bla IMP-4 (1,008)+382 (intI1 ATG) to + 474 (blaIMP-4 ATG)Combining with RFLP for detection of the intI1-blaIMP-4 sequenceaThis work
int1b aac61bBCAT CCA AGC AGC AAG CGC GTT A ACC CCG GIT TCT CGT AGC AT5′ CS-arr-2-aacA4 (1,182)−131 (arr-2 ATG) to +460 (aacA4 ATG)Combining with RFLP for the detection of the 5′ CS-arr-2-aacA4 sequenceaThis work
int1e int1cTCG TAG AGA CGT CGG AAT GG CCG AGG CAT AGA CTG TAC AAClass 1 integrase gene intI1 (965)−40 to +925 (intI1 ATG)For detection of the intI1 gene 27
5cs 3csGGC ATC CAA GCA GCA AG AAA GCA GAC TTG ACC TGAEntire integron (variable)VariableFor confirmation of the presence of class 1 integron 27
ardra1 ardra2TGG CTC AGA TTG AAC GCT TAC CTG TTA CGA CTT CA16S rRNA gene (1,500)VariableARDRA of acinetobacter for genomic DNA group determination 11
BL BRTAT GAG TGG CTA AAT CGA T CCC GCT TTC TCG TAG CA aacA4 (395)+115 to +509 (aacA4 ATG)For detection of the aacA4 gene 39
aph1 aph2ATA CAG AGA CCA CCA TAC AGT GGA CAA TCA ATA ATA GCA AT aph(3′)-VIa (234)+140 to +374 [aph(3′)-VIa ATG]For detection of the aph(3′)-VIa gene 60
  • a See Table 2 for the expected sizes of the restriction fragments.