TABLE 1.

CYP51A mutations and expression levels of AfuMDR3 and AfuMDR4 in RIT mutant strains

StrainAfuMDR3 cDNA levelAfuMDR4 cDNA levelCYP51A mutation
Constitutively expressed strainaItraconazole-treated strainbConstitutively expressed strainaItraconazole-treated strainb
H11-201.0 (0.8-1.2)1.3 (0.6-2.7)1.0 (0.8-1.2)2.2 (1.9-2.6)
RIT12.4 (0.8-7.1)0.5 (0.2-1.4)1.4 (0.8-2.5)1.4 (0.8-2.4)G54E
RIT229.9 (27.7-32.2)3.2 (3.2-3.3)5.2 (5.0-5.3)2.8 (2.6-3.0)G54E
RIT330.9 (26.6-35.9)1.0 (0.9-1.1)6.8 (5.8-8.1)1.2 (0.5-3.2)G54E
RIT41.9 (0.4-8.9)0.8 (0.3-1.9)1.7 (0.8-3.6)0.7 (0.4-1.1)
RIT5121.5 (84.7-174.4)1.1 (0.8-1.4)14.7 (10.3-21.0)1.1 (1.0-1.2)
RIT60.6 (0.1-2.5)2.1 (0.9-5.0)1.5 (1.0-2.3)1.2 (0.7-2.0)
RIT817.7 (6.3-50.0)0.6 (0.3-1.5)2.1 (1.1-3.9)0.8 (0.6-1.2)
RIT90.7 (0.3-2.0)0.8 (0.2-2.6)1.3 (0.6-3.2)0.3 (0.1-1.4)
RIT1036.3 (9.8-134.6)0.6 (0.6-0.7)9.4 (2.9-31.3)0.4 (0.4-0.4)
RIT1120.7 (13.6-31.5)4.8 (3.4-6.8)1.6 (1.0-2.4)11.2 (7.6-16.5)
RIT122.7 (2.0-3.8)2.4 (0.6-8.7)4.1 (3.0-5.7)0.6 (0.3-1.0)G54E
RIT131.5 (1.2-1.8)139.6 (83.0-234.7)1.0 (0.9-1.2)60.8 (37.4-98.8)G54E
RIT149.9 (2.0-48.3)0.6 (0.2-1.9)1.2 (0.6-2.8)2.4 (1.7-3.6)
RIT152.4 (0.6-10.0)1.9 (0.7-4.7)1.1 (0.5-2.5)0.5 (0.3-1.0)G54K
RIT166.4 (3.6-11.2)0.5 (0.2-1.5)2.6 (1.3-5.0)0.3 (0.2-0.6)
RIT184.7 (1.7-12.9)2.3 (0.5-10.2)3.1 (1.9-5.1)2.9 (2.2-4.0)G54K
RIT191.2 (0.5-3.2)2.6 (1.0-6.9)2.4 (4.9-1.1)1.1 (0.4-2.7)G54R
RIT200.1 (0.0-0.4)3.2 (0.4-23.7)1.2 (0.3-4.5)1.3 (0.5-3.2)
RIT210.8 (0.1-6.3)3.6 (1.2-11.0)0.4 (0.1-2.7)1.2 (0.2-7.6)
RIT220.2 (0.0-0.6)1.9 (0.9-4.2)0.3 (0.1-1.3)1.1 (0.4-3.4)
RIT232.6 (0.3-19.2)2.8 (0.3-24.8)0.5 (0.1-2.7)1.4 (0.2-8.5)
RIT240.04 (0.002-0.9)29.3 (8.6-100.2)0.3 (0.1-1.0)2.0 (0.8-4.9)
RIT262.8 (0.8-9.9)1.4 (0.4-5.3)0.4 (0.1-0.9)0.3 (0.04-2.6)
  • a cDNA levels were calculated relative to that of the untreated parental strain H11-20. The numbers represent the averages of 3 to 5 replicates, with the ranges shown in parentheses.

  • b Values were obtained by using the cDNA copy number of each respective untreated strain as the baseline for strains in the presence of drug following normalization based on β-tubulin cDNA copy number. Cells were induced with 10 (H11-20) or 100 μg of itraconazole ml−1 for 8 h. Standard deviations are in parentheses.