TABLE 1.

Candida albicans susceptibilitiesa

StrainGeometric mean MIC (μg/ml)b
FluconazoleVoriconazoleCaspofunginMicafungin
ATCC 900280.960.030.050.06
DAY185 (control strain)0.620.020.060.03
vps28−/0.390.010.020.01
vps32−/0.320.010.020.01
vps28−/+VPS280.620.030.030.04
vps32−/+VPS320.680.020.030.04
DAY286+RIM101SL0.750.030.040.02
vps28−/+RIM101SL0.750.020.0070.001
vps32−/+RIM101SL0.620.010.0050.001
rim101−/0.110.0020.0050.001
rim101−/+RIM101SL0.420.010.030.03
  • a Calculated from at least 10 independent experiments. All strains derive directly from BWP17 (His Ura Arg) (4) and are prototrophic. Double disruptions restored a Ura+ Arg+ phenotype. The His+ information was brought by the plamsids carrying VPS28, VPS32, or RIM101SL or by an empty vector, all integrated at HIS1. DAY286 is an Arg+ Ura+ derivative of DAY185.

  • b Results are MIC minus 2 (prominent growth reduction or a 50% reduction in optical density) at 24 h for CAS and MCF and at 48 h for FLC and VRC.