TABLE 1.

Strains and plasmids used in this study

Strain or plasmidRelevant feature(s)cSource or reference
S. aureus
    COLHomogeneous Mcr Ems; MIC,a 400 μg/mlRU collection
    RN4220Mcs, restriction negativeR. Novick
    COLVA200Strain COL carrying a plasmid-borne Tn1546 vanA gene complex 26
    COLspac::pbpBCOL with Pspac-pbpB fusion in the chromosome transformed with pMGPII 24
    COL ΔvraSCOL/pSG4 inserted into the chromosome, EmrThis study
    COLVA200ΔvraSCOLVA200/pSG4 inserted into the chromosome, EmrThis study
    301Homogeneous Mcr Ems; MIC,a 200 μg/mlUnited States
    301ΔvraS301/pSG4 inserted into the chromosome, ErmrThis study
    DU4916Homogeneous Mcr Ems; MIC,a 200 μg/ml 8
    DU4916ΔvraSDU4916/pSG4 inserted into the chromosome, ErmrThis study
    319Homogeneous Mcr Ems; MIC,a 100 μg/mlItaly, 1990
    319ΔvraS319/pSG4 inserted into the chromosome, ErmrThis study
    404Homogeneous Mcr Ems; MIC,a 100 μg/mlItaly, 1990
    404ΔvraS404/pSG4 inserted into the chromosome, ErmrThis study
    E3971Homogeneous Mcr Ems; MIC,a 100 μg/ml 6
    E3971ΔvraSE3971/pSG4 inserted into the chromosome, ErmrThis study
    RUSA11RN2667 transformed with SCCmec type I from strain COLRU collection (17)
    RUSA11ΔvraSRUSA11/pSG4 inserted into the chromosome, ErmrThis study
    ZOX3Laboratory step mutant, Mcs Czr; MIC,b 6 μg/ml, 15
    ZOX12Laboratory step mutant, Mcs Czr; MIC,b 24 μg/ml 15
    ZOX3ΔvraSZOX3/pSG4 inserted into the chromosome, ErmrThis study
    ZOX12ΔvraSZOX12/pSG4 inserted into the chromosome, ErmrThis study
    Sanger 476Mcs, hypervirulent strainUnited Kingdom (11)
    Sanger 476ΔvraS476/pSG4 inserted into the chromosome, ErmrThis study
    Sanger 476 mecASanger 476 containing plasmid pSTP181-borne mecA gene from strain COL; MIC,a 0.75 μg/ml; MIC,b 24 μg/mlUnpublished data
    Sanger 476 mecAΔvraSSanger 476 mecA/pSG4 inserted into the chromosome, ErmrThis study
E. coli DH5α recA endA1 gyrA96 thi-1 hsdR17 supE44 relA1 φ80 ΔlacZΔM15Bethesda Research Laboratories
Plasmids
    pSP64EIntegrational vector for S. aureus, Apr Emr 23
    pSG4pSP64E/637pb internal DNA fragment of vraS geneThis study
  • a Oxacillin MIC.

  • b Ceftizoxime MIC.

  • c Apr, ampicillin resistant; Emr, erythromycin resistant; Cmr, chloramphenicol resistant, Czr, ceftizoxime resistant.