TABLE 1.

Bacterial strains and plasmids used in this study

Plasmid or strainRelevant characteristicsaSource or reference
Plasmids
    pCR2.1Apr; PCR cloning vectorInvitrogen
    pUCP20Apr; E. coli-P. aeruginosa shuttle vector14
    pPS1679Apr; pUCP20 containing the bpeEF-oprC operon of B. pseudomallei 1026bThis study
    pUC18-mini-Tn7T-LACApr Gmr; mini-Tn7 expression vector containing lacIq and tac promoter3
    pPS1738Apr Gmr; pUC18-mini-Tn7-LAC containing the bpeEF-oprC operon of B. pseudomallei 1026bThis study
    pEXGm-ΔpscCApr; gene replacement vector containing unmarked P. aeruginosa ΔpscC allele15
    pTNS2Apr; helper plasmid encoding the site-specific TnsABCD Tn7 transposition pathway3
    pFLP2Apr; source of Flp recombinase7
P. aeruginosa strains
    PAO397Δ(mexAB-oprM) Δ(mexAB-oprM) Δ(mexCD-oprJ) Δ(mexEF-oprN) Δ(mexJK) Δ(mexXY) ΔopmH6
    PAO750PAO397 with ΔpscCThis study
    PAO783Gmr; PAO750 with chromosomally integrated mini-Tn7T-LAC-bpeEF-oprCThis study
    PAO789PAO783 without the Gmr markerThis study
  • a Abbreviations: Ap, ampicillin; Gm, gentamicin; r, resistance.