TABLE 1.

P. aeruginosa strains and plasmids

Strain or plasmidDescriptionSource or reference
Strains
    PAO1Wild type31
    PAO-MW1PAO1 rhlI::Tn501 lasI::tetA45
    PAO lasR rhlRlasR::Tcr ΔrhlR::Gmr) of PAO131
Plasmids
    pQF50Broad-host-range plasmid for promoter analysis10
    pMW312pQF50 carrying the rsaL promoter from −82 to +29 relative to the translation start of rsaL; Apr44
    pUC18Cloning vector49
    pUM11yfp with T7gene10 ribosome binding site in pUC18This study
    pUM15rsaL::yfp transcriptional fusion, derived from pUM11 and pMW312; AprThis study
    pRSET(B)Expression vectorInvitrogen Corporation
    pRSET(B)-10Bnhyfp cloned into pRSET(B)This study