TABLE 1.

Strains used in this study and tested for persisters

StrainGenotypeBiofilm architecturePersistersaSource/reference
3153AWild-type laboratory strainRobust 3D wild type+++41
CKY357CAI-4 mkc1Δ::hisG/mkc1Δ::hisG mkc1::pCK70 (URA3)Reduced filamentation++26
CAI4SC5314 Δura3::λimm434/Δura3::λimm434Robust 3D wild type+26
CKY136CAI-4 efg1::hisG/efg1::hisG ade2::pDBI52 (URA3)Filamentation defect; sparse monolayer of cells+++12
CKY138CAI-4 efg1::hisG/efg1::hisG cph1Δ::hisG/cph1Δ::hisG ade2::pDBI52 (URA3)Filamentation defect; sparse monolayer of cells++12
MC191ura3Δ::λimm434/ura3Δ::λimm434 arg4::hisG/arg4::hisG his1::hisG/his1::hisG flo8::ARG4/flo8::HIS1 ade2::URA3/ADE2Functionally defective hyphae+++Microbia
MC195ura3Δ::λimm434/ura3Δ::λimm434 arg4::hisG/arg4::hisG his1::hisG/his1::hisG flo8::ARG4/flo8::HIS1 ade2::URA3::FLO8-2/ADE2Robust 3D wild type+Microbia
MC245ura3Δ::λimm434/ura3Δ::λimm434 arg4::hisG/arg4::hisG his1::hisG/his1::hisG flo8::ARG4/FLO8 ade2::URA3/ADE2 HIS::his/hisRobust 3D wild type++Microbia
DAY185Δura3::λimm434ura3::λimm434 arg4::hisG/arg4::hisG/pARG4-URA3 his1::hisG/his1::hisG/pHIS1Robust 3D wild type++45
DAY286Δura3::λimm434/Δura3::λimm434 arg4::hisG/arg4::hisG/pARG4-URA3 his1::hisG/his1::hisGRobust 3D wild type++45
GKO443Δura3::λimm434/Δura3::λimm434arg4::hisG/arg4::hisG his1::hisG/his1::hisG suv3::Tn7-UAU1/suv3::Tn7-URA3Biofilm defect; decreased biomass++45
GKO798Δura3::λimm434/Δura3::λimm434arg4::hisG/arg4::hisG his1::hisG/his1::hisG kem1::Tn7-UAU1/kem1::Tn7-URA3Biofilm defect; decreased biomass++37
GKO814Δura3::λimm434/Δura3::λimm434arg4::hisG/arg4::hisG his1::hisG/his1::hisG nup85::Tn7-UAU1/nup85::Tn7-URA3Biofilm defect; decreased biomass++45
GKO9Δura3::λimm434/Δura3::λimm434arg4::hisG/arg4::hisG his1::hisG/his1::hisG mds3::Tn7-UAU1/mds3::Tn7-URA3Biofilm defect; decreased biomass++45
CJN702Δura3::λimm434/Δura3::λimm434arg4::hisG/arg4::hisG his1::hisG::pHIS1/his1::hisG bcr1::ARG4/bcr1::URA3Functionally defective hyphae++37
CJN698Δura3::λimm434/Δura3::λimm434arg4::hisG/arg4::hisG his1::hisG::pHIS1-BCR1/his1::hisG bcr1::ARG4/bcr1::URA3Robust 3D wild type++37
  • a +++, 1 to 2% survival; ++, 0.1 to 1% survival; +, 0.05 to 0.1% survival.