TABLE 2.

Nucleotide sequences of primers used for amplification and probes used for real-time PCR

Target or probeSequencePositionaDirectionb
Target
    rplF5′-TCTAAACCAGGTTTACG-3′205402F
5′-ACTCAGCAGTACCAGAG-3′206354R
    Ef16SA5′-AAGTTCTTGACATTCGAACGA-3′248256F
5′-GGTGTTCTCGGTTTGTTTTG-3′250129R
    Ef16SB5′-AGACGACAACGACCGAGCGG-3′1017931F
5′-AAGGCCCCGAATTTATTTGA-3′1019808R
    Ef16SC5′-AAGGCCCCGAATTTATTTGA-3′2771761F
5′-CAGACAACAACAACCAAGCAA-3′2773637R
    Ef16SD5′-GGTGTTCTCGGTTTGTTTTG-3′3168355F
5′-CAGACAACAACAACCAAGCAA-3′3170274R
    Ef16Sc5′-ACGATCCGAAAACCTTCTTCAC-3′2772962R
5′-TCGGCAATGGACGAAAGTC-3′2773023F
    atp5′-CTGCCAACTCAAACAAAGCCT-3′2528854F
5′-GGTACGATAGCTTTGCCAGGG-3′2530619R
    atpEc5′-GACACCTAAAATTGGCACAGCTT-3′2529599R
5′-TGTCTGGTCAATTAAGAACAACAATG-3′2529669F
    atpBc5′-TCGGCGGTTGGACTTTTC-3′2530221R
5′-CTTGGGTCTCGTTACAAAAATCG-3′2530286F
Probec
    atpE5′-ATTAAAGCGACCCCGATAA-3′2529624F
    atpB5′-AAACGCTGAGATCGTCGCCCACA-3′2530240F
    Ef16S5′-CACGCGGCGTTGCTCGGTC-3′2772995F
  • a Corresponding E. faecalis V583 position.

  • b F, forward; R, reverse.

  • c Used for real-time PCR.