TABLE 2.

Ergosterol contents, p values, and passive diffusion of R6G in untreated and BPS- and BCS-treated wild-type cells and mutant cells with defective iron uptake

Strain (condition for growth)% Ergosterolap valuebOD527c
CAI41.22 ± 0.050.171 ± 0.0010.309 ± 0.023
CAI4 (200 μM BPS)0.81 ± 0.020.08 ± 0.010.209 ± 0.02
CAI4 (500 μM BCS)1.08 ± 0.040.113 ± 0.0080.202 ± 0.033
Δftr10.97 ± 0.020.103 ± 0.010.237 ± 0.019
Δccc21.03 ± 0.0330.114 ± 0.010.202 ± 0.02
  • a The mean ergosterol content of cells is expressed as a percentage of the wet weight of cells ± the standard deviation of three sets of experiments (33).

  • b The mean p value of cells (inversely proportional to membrane fluidity)± the standard deviation of three sets of experiments is shown (32, 33, 35).

  • c The mean OD527 of supernatant, for checking passive diffusion of R6G inside cells (a lower OD implies a higher concentration of R6G inside the cells), ± the standard deviation of three sets of experiments is shown (32, 33, 35).