TABLE 1.

Conjugation with J53Azr and selection of transconjugants

DonorMating conditionAntimicrobials used for selectionaConjugation frequencybCoresistance ratecTransferred plasmid size (kb)
4LiquidAZD + CHL1.6 × 10−496.4>180 (4-3), 85 (4-59, pHSH1), 54 (4-62)
7LiquidAZD + CHL1 × 10−4100>180
10LiquidAZD + TET4 × 10−396.368
12LiquidAZD + TET6 × 10−783.368 (pHSH2)
29LiquidAZD + TET5 × 10−410068
76FilterAZD + GEN1 × 10−21.3>180
  • a AZD, azide; CHL, chloramphenicol; GEN, gentamicin; TET, tetracycline.

  • b Conjugation frequency = number of transconjugants/number of donor cells.

  • c The coresistance rate is the percentage of selected colonies for which the MIC of ciprofloxacin increased.