TABLE 2

Mutations repeatedly observed in cultures of strain PA83a

GeneLocus tagMutation(s) in:
PAO1PA83v02v03v05v06v08v11v12v14v15
lpxCPA4406PSMA83_05042P101SV222A, S106GV222AV164G, A107TA107T, G21W, F176SA107T, I131FM103ID232E, D232G, V217F, V217AV222A, S106G
pmrBPA4777PSMA83_05449L96RL171PL87PF51LS8P, E320KV9AG123SE320K, A248T, L167PR259H, V361M
Gene for putative transferasePA3853PSMA83_01172C226GY3C, G62SV34A, Y155CC226GR60C, Y216C, E185GC226GV122A, E185G
Gene for asparagine synthetasePSMA83_05677L365PFrameshiftL425PG32SFrameshiftW153XL365P, W153X V286M
migAPA0705PSMA83_04868H219PC25R, N27SD106GQ191R, V22AT196P, H123PH219PA168T
mutSPA3620PSMA83_01420T51PT51PT51PT51PT51PT51P, T287P
lpxO2PA0936PSMA83_04590D163AD163NW209XD163AFrameshiftIn-frame deletion
pmrAPA4776PSMA83_05448L11QL11PR159L, G15V, N172D
Gene for putative outer membrane proteinPA3647PSMA83_01392K122XFrameshiftV1V, R2G
Gene for paraquat-inducible proteinPSMA83_05349G180XFrameshift
1PA4773PSMA83_05445W35XFrameshift
cupB5PA4082PSMA83_00934G260X, R26CP139P
2PA5133PSMA83_05845FrameshiftFrameshiftFrameshift
pdtAPA0690PSMA83_04884A3885V, A3885AG1527X
morAPA4601PSMA83_05248R1199HG143D
lpxAPA3644PSMA83_01395R96SR191C
priAPA5050PSMA83_05733L38LR689R
Gene for putative chemotaxis transducerPA4633PSMA83_05281L315PA260A
Gene for putative lipoproteinPA3069PSMA83_01992K83RL68P
traNPSMA83_06409W773XG912D
Gene for polyketide synthase type IPSMA83_02547I876MV781V
3PA0242PSMA83_00250S420GA579A
Gene for hypothetical proteinPA4782PSMA83_05455P38SP38P
wbpMPA3141PSMA83_01912E273KE273G
mscLPA4614PSMA83_05261V86IS35P
  • a The complete list of mutations, including annotation and locus tag of each gene, is available in Data Set S4. For genes with long names, the table lists the PAO1 locus tag where available; the annotations of these genes are as follows: 1, putative S-adenosylmethionine decarboxylase proenzyme; 2, putative membrane-bound metallopeptidase; and 3, putative 4-hydroxyphenylpyruvate dioxygenase.