TABLE 5.

Fold changes in MICs of quinolones for GyrA and GyrB mutants relative to MICs for DM200 parental strain

StrainGyrA mutationGyrB mutationFold change in MIC relative to MIC for DM200a
PGE- 8367769 (0.25)bPGE-6596491 (0.5)PGE-7872411 (0.25)PGE-878935 (0.125)PGE-5215205 (64)PGE-9604297 (8)Nalidixic acid (0.5)Cipro- floxacin (0.004)Clina- floxacin (0.002)
DM289E16V888421111
DM291G31V448421111
DM276R38L448821111
DM275G40A444411−2−21
DM279Y50D44442−21−2−2
DM283V70Ac488822211
DM225G81Cc−21≤−4≤−21166484
DM218S83Lc16321616241281616
DM344S83Wc1616816281283216
DM270A84Vc488814114
DM219D87Gc4484223284
DM221D87Yc2242226484
DM232D87Nc4444226488
DM237D87Hc4488223284
DM280I89Lc444424111
DM281M135T448824111
DM282G173S844811211
DM290T180I448824122
DM271F217C448428112
DM288P218T448824211
DM293F513C888411211
DM327D426N412211822
DM316G429V164≤−4−241424
DM236K447N21−211164≤−4−2
DM318E466D4484≥4−2444
  • a Fold change was calculated by dividing the MIC for the mutant strain by the MIC for strain DM200. For ratios less than 1, the negative reciprocal is displayed. Changes of fourfold or greater are in boldface, and fold changes of negative fourfold or less are in italics.

  • b The values in parentheses are the MICs for DM200 (in micrograms per milliliter).

  • c Residues inside the GyrA QRDR.