TABLE 1.

Bacterial strains used in this study

StrainGenotype or descriptionSource or reference
E. coli
    ATCC 25992Quality control strain (FDA strain Seattle 1946)ATCCa
    D21proA23 lac-28 tsx-81 trp-30 his-51 rpsL173 (Strr) tufA1 ampCp-123
    D22D21 lpxC10123
    CS1562tolC6::miniTn1025
    DM200D21 tolC6::mini-Tn10 [P1(CS1562) × D21]This study
    DM202D22 tolC6::mini-Tn10 [P1(CS1562) × D22]This study
    BW25113/pKD46Δ(araD-araB)567 ΔlacZ4787(::rmB-4) laclp-4000(lacIq) λ rpoS396(Am) Δ(rhaD-rhaB)568 rmB-4 hsdR5147
    CAG12183 gyrA+λ zfa-3145::Tn10kan rph-125
    DM232DM200 gyrA (D87N)Macingab
    DM236DM200 gyrB (K447N)Macingab
    DM237DM200 gyrA (D87H)Macingab
    DM316DM200 gyrB (G429V)Macingab
    DM318DM200 gyrB (E466D)Macingab
    DM327DM200 gyrB (D426N)Macingab
    DM340BW25113 gyrA (S83W)This study
    DM344DM200 gyrA (S83W) [P1(DM340 × DM200]This study
K. pneumoniae KL618Respiratory pathogenMRL Pharmaceutical Services
M. catarrhalis BC24UCLAc Medical Center throat isolate (strain UCLA 77)J. Hindler
S. aureus ATCC 29213Quality control strainATCC
S. pneumoniae ATCC 49619Quality control strainATCC
S. pyogenes STA2Chenango Memorial Hospital isolate (strain 1016)Norwich, N.Y.
  • a ATCC, American Type Culture Collection.

  • b D. R. Macinga, unpublished data.

  • c UCLA, University of California at Los Angeles.