TABLE 4.

Amino acid substitutions and mutations identified in AcrRa

StrainDNA changeAmino acid and codon substitution or mutation
S67 aab substitutionsP160R, G163A, F171S, R172G, L194V, F196I, K200M
S8, S30, S377 aa substitutions, one silent changeP160R, G163A, F171S, R172G, L194V, F196I, K200M, L186L (CTG→CTT)
S28, S297 aa substitutions, three silent changesP160R, G163A, F171S, R172G, L194V, F196I, K200M, E146E (GAA→GAG), L186L (CTG→CTT), L197L (CTG→TTG)
KP38 aa substitutionsA20T, P160R, G163A, F171S, R172G, L194V, F196I, K200M
S511-bp deletion, frameshift mutationΔ144-148, TLKE
S1011-bp deletion, frameshift mutationΔ144-148, TLKE
S1311-bp deletion, frameshift mutationΔ144-148, TLKE
S3611-bp deletion, frameshift mutationΔ144-148, TLKE
S7c15-bp deletionΔ128-132, QAQRQ
  • a In comparison with the K. pneumoniae AcrR sequence in the GenBank database (GenBank accession no. AJ318073).

  • b aa, amino acid.

  • c S7 has the same amino acid substitutions as S6.