TABLE 2.

Primers and annealing temperatures used in the study

GenePrimerTm (°C)aGenBank Accession no.
KpgyrA15′-TGCGAGAGAAATTACACC-3′56X16817
KpgyrA25′-AATATGTTCCATCAGCCC-3′
KpmarR15′-CCAGCGACCTGTTTAATGA-3′56M96235
KpmarR25′-GCGTCATTATTACGTCTGG-3′
KpmarA15′-TGCTCAAGAAGGTCCTGCC-3′58M96235
KpmarA25′-TGCGGCAGCGAATAGTTTC-3′
KpsoxS15′-CCCATCAGGATATTATTCA-3′52U00734
Kpsox25′-AGATGTGATGGCGATAGT-3′
KpacrE15′-ATGACGACTCACGCCA-3′50M96848
KpacrE25′-CGTTTCACCGTCAAATG-3′
KpramA15′-GGGTCGCCGATAAGACGC-3′60U19581
KpramA25′-GCTGGGCGCCATTGAGTAT-3′
Kpramop15′-TATCAACGGCTGGCGGCT-3′58U19581
Kpramop25′-GCAGCGGTTGATGCAGGT-3′
KpacrRNru1b5′-TGAGTCGCGAATTAAGCTGACAAGCTCTC-3′58AJ318073
KpacrRBcl1c5′-TGAGTGATCAGGTCATGCTATGGTACATA-3′
KpacrA15′-ATGAACAAAAACAGAGG-3′52AJ318073
KpacrA25′-TTTCAACGGCAGTTTTCG-3′
  • a Tm, melting temperature.

  • b Underlining indicates the Nru1 site.

  • c Underlining indicates the Bcl1 site.