TABLE 1.

Reference strains and plasmids used in this study

Strain or plasmidRelevant genotype or phenotypeSource or reference
Strains
    E. coli JM109 endA1 gyrA96 hsdR17 Δ(lac proA) relA recA1 supE44 thi F (lacIqZΔM15 proAB+traD36)Gibco BRL-Life Technologies
    E. coli DH10B araD139 Δ(ara leu)7697 deoR endA1 galK1 galU nupG recA1-rpsL F′ mrcA Δ(mrr-hsdRMS-mrcBC)Φ80dlacZΔM15 ΔlacX74Stratagene
Plasmids
    pPCRScript Amp SK(+)Ampicillin resistantStratagene
    pACYC184Chloramphenicol resistant, low copy number 11
    pBK-CMVKanamycin resistant, high copy numberStratagene
    pILT-3Natural ca. 50-kb plasmid of K. pneumoniae ILT-3 producing CTX-M-19 39
        β-lactamase
    pA-1Recombinant pPCR-Script Amp with a 6.5-kb PCR fragment from whole-cell DNA of K. pneumoniae ILT-3 containing ISEcp1B and a truncated blaCTX-M-19 geneThis study
    pB-1Recombinant pPCR-Script Cam with a 1.1-kb PCR fragment from whole-cell DNA of K. pneumoniae ILT-3 containing ISEcp1B promoter sequences and the entire blaCTX-M-19 geneThis study
    pB-2Recombinant pPCR-Script Cam with a 1-kb PCR fragment from whole-cell DNA of K. pneumoniae ILT-3 lacking ISEcp1B-promoting sequences and containing the entire blaCTX-M-19 geneThis study