TABLE 1.

Bacterial strains and plasmids used or identified in the study

Species and strain or plasmidaRemarksSource or referenceb
S. intermedius
    119-rWild-type strain, pNVH01This study, A
    139-rWild-type strain, pNVH01This study, B
    139-sClosely related to 139-r, but plasmid freeThis study, B
S. aureus
    120-rWild-type strain, pNVH01This study, A
    120-cIsogenic to 120-r but cured for plasmidThis study, A
    140-rWild-type strain, pNVH01This study, B
    140-sIsogenic to 140-r but plasmid freeThis study, B
    538-rWild-type strain, pNVH01This study, C
    538-cIsogenic to 538-r but cured for plasmidThis study, C
    184-rWild-type strain, pNVH01This study, D
    RN4220Restriction-deficient, plasmid-free host strain12
    RN4220(pSK265)RN4220 containing cloning vector pSK26511
    RN4220(pSK265) smrRN4220 containing smr gene and promoter8
    RN4220(pSK265) qacGRN4220 containing qacG gene and promoter8
    RN4220(pSK265) qacHRN4220 containing qacH gene and promoter7
    RN4220(pSK265) qacJRN4220 containing qacJ gene and promoterThis study
S. simulans 138-rWild-type strain, pNVH01This study, B
E. coli DH5αHost strain for pUC18Life Technologies
Plasmids
    pNVH012.650-kb; contains qacJThis study
    pUC18 SmaI/BAP2.686-kb cloning vectorAmersham Biosciences
    pSK2653.0 kb cloning vector11
    pSK265/qacJ3.6 kb with qacJ gene and promoterThis study
  • a r, QAC resistant; s, QAC sensitive; c, cured for pNVH01.

  • b A, B, C, and D refer to the horses from which the strains were isolated.