TABLE 2.

Laboratory detection of hVISA

Laboratory (reference)Detection methoda
Simplified PAP on BHIA-V4Simplified PAP on MHABroth microdilution (MIC)Agar dilution (MIC)EtestPAPDisk-agarMicroScanResistant mutant emergence
Hiramatsu et al. (19)I
Franchi et al. (11)IC*
Ariza et al. (1)XX
Kantzanou et al. (23)I♣CC
Geisel et al. (14)I
Wong et al. (39)II
Bierbaum et al. (2)IC
Schmitz et al. (31)XXXX
Marchese et al. (26)Iδ♣C
Kim et al. (24)IC
Bobin-Dubreux et al. (3)CCC
Reverdy et al. (30)CCCI
Trakulsomboon et al. (37)CCC
Wootton et al. (41)XXXX
  • a Disk-agar, aztreonam disk on vancomycin salt; MicroScan, Microscan conventional gram-positive panels; I, initial screening test; C, confirmatory test; X, for the tests performed, did not distinguish between initial and confirmatory tests; *, PAP performed on MHA; Ψ, MIC determined on subclone recovered from initial screen; δ, 100 μl of starting cell suspension (instead of 10 μl) plated; φ, MHA with 2 μg of vancomycin per ml; ♣, McFarland standard of 1.0 (instead of 0.5) used.