TABLE 3.

Ex vivo effects of d4T, AZT, and BAIBA on [U-14C]palmitic acid β-oxidation in liver mitochondriaa

Treatment (dose)MiceAcid-soluble β-oxidation products (nmol/mg of protein/min)
Total activityMitochondrial activityPeroxisomal activity
d4T (13.5 mg/kg/day)Control18.0 ± 4.116.0 ± 3.32.0 ± 0.5
Treated20.6 ± 4.418.5 ± 3.92.1 ± 1.4
AZT (100 mg/kg/day)Control15.4 ± 1.412.7 ± 1.22.7 ± 0.4
Treated19.3 ± 1.616.7 ± 1.6b2.6 ± 0.5
BAIBA (100 mg/kg/day)Control20.3 ± 3.616.8 ± 2.73.5 ± 1.5
Treated33.8 ± 8.4b31.6 ± 2.9b1.9 ± 1.5
  • a Mice were treated or not with d4T, AZT, or BAIBA for 2 weeks and fasted for the last 48 h of treatment. Liver mitochondria were isolated and incubated at 30°C for 10 min with [U-14C]palmitic acid, ATP, coenzyme A, and carnitine, with or without 2 mM KCN. The mitochondrial activity was determined as the difference between the β-oxidation activity measured without KCN (total activity) and that with KCN (peroxisomal activity). Results are means ± SEMs for eight mice.

  • b The value is significantly different from the value for the corresponding control at a P of <0.05.